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化粧品中安息香酸デナトニウムの分析と誤飲防止効果
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森謙一郎、中村義昭、大貫奈穂美、寺島 潔、横山敏郎、宮本道子、荻野周三、斉藤和夫
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東京都健康安全研究センター医薬品部微量分析研究科
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Determination of denatonium benzoate in cosmetics and their antifeeding property
Ken'ichiro MORI Yoshiaki NAKAMURA Nahomi OHNUKI Kiyoshi TERAJIMA Toshiro YOKOYAMA Michiko MIYAMOTO Shuzo OGINO Kazuo SAITO
A method for the determination of denatonium benzoate benzyldiethyl-N-26-xylylcarbamoylmethylammonium benzoate abbreviated to DB in cosmetics was developed. A cosmetic sample of 1-2 g was weighed and dissolved with methanol up to a volume of 10 mL. When a precipitate was seen it was cleared using a high speed centrifuge. The solution was loaded on a cation exchange column washed with methanol and eluted by 1% HCl-MeOH 10 mL. The eluate was evaporated to dryness dissolved by 0.1% HCl-MeOH 500 µL and injected on an HPLC. The conditions for HPLC were as follows: column ODS mobile phase water/acetonitrile (7 : 3) containing 0.01 mol/L sodium 1-pentanesulfonate and 0.015 mol/L sodium sulfate injection volume 20 µL detector 206 nm. The detection limit was 0.1 ppm. By this method 1.0 to 9000 ppm of DB were detected in five imported cosmetics without any interference. A 1.0 mL of 1.0 ppm DB water solution fully convinced us of DB's antifeeding property because of the intense bitter taste. We have rarely seen Japanese cosmetics or household articles containing DB as aversive agents to prevent accidental ingestion but there are many and wide ranges in US or European articles.
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| Keywords: |
化粧品 , 安息香酸デナトニウム , 誤飲防止剤 , 忌避剤 , 液体クロマトグラフィー , |
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[PDF (305.8K)]
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第4巻 , 第1号 , (pp.26-33)
